Glutamyl aminopeptidase in microvesicular and exosomal fractions of urine is related with renal dysfunction in cisplatin-treated rats.

Andrés QuesadaAna Belén SegarraSebastián Montoro-MolinaMaría Del Carmen de GraciaAntonio OsunaFrancisco O'ValleManuel Gómez-GuzmánFélix VargasRosemary Wangensteen

Published in: PloS one (2017)

Determination of GluAp content or its enzymatic activity in microvesicular and exosomal fractions of urine is an early and predictive biomarker of renal dysfunction in cisplatin-induced nephrotoxicity. Measurement of GluAp in these fractions can serve to detect proximal tubular damage independently of glomerular filtration status.

Keyphrases

oxidative stress
high glucose
hydrogen peroxide
solid phase extraction
diabetic nephropathy
mass spectrometry
high resolution
liquid chromatography
tandem mass spectrometry