Quantitative protein mass-spectrometry requires a standardized pre-analytical phase.
Nico P M SmitFred P H T M RomijnVanessa J J van HamEsther ReijndersChrista M CobbaertL Renee RuhaakPublished in: Clinical chemistry and laboratory medicine (2022)
Plasma anticoagulants affect QPMS test results. Heparin plasma showed comparable results to serum. Reduced concentrations in citrate plasma can be explained by dilution, whereas reduced recovery in EDTA-plasma is dependent on altered proteolytic digestion efficiency. The results highlight the importance of a standardized pre-analytical phase for accurate QPMS applications in clinical chemistry.