Wound infections, especially those caused by pathogenic bacteria, present a considerable public health concern due to associated complications and poor therapeutic outcomes. Herein, we developed antibacterial nanoparticles, namely, PGTP, by coordinating guanidine derivatives with a porphyrin-based sonosensitizer. The synthesized PGTP nanoparticles, characterized by their strong positive charge, effectively disrupted the bacterial biosynthesis process through charge interference, demonstrating efficacy against both Gram-negative and Gram-positive bacteria. Additionally, PGTP nanoparticles generated reactive oxygen species under ultrasound stimulation, resulting in the disruption of biofilm integrity and efficient elimination of pathogens. RNA-seq analysis unveiled the detailed mechanism of wound healing, revealing that PGTP nanoparticles, when coupled with ultrasound, impair bacterial metabolism by interfering with the synthesis and transcription of amino acids. This study presents a novel approach to combatting wound infections through ultrasound-driven charge-interfering therapy, facilitated by advanced antibacterial nanomaterials.
Keyphrases
- gram negative
- wound healing
- rna seq
- multidrug resistant
- magnetic resonance imaging
- public health
- reactive oxygen species
- solar cells
- ultrasound guided
- contrast enhanced ultrasound
- amino acid
- pseudomonas aeruginosa
- transcription factor
- walled carbon nanotubes
- photodynamic therapy
- computed tomography
- risk factors
- silver nanoparticles
- biofilm formation
- anti inflammatory
- metabolic syndrome
- insulin resistance
- mesenchymal stem cells
- bone marrow
- data analysis
- cell therapy