Effects of tetrahedral DNA nanostructures on the treatment of osteoporosis.
Weitong CuiXiao YangYikai DouYue DuXiaohong MaLei HuYunfeng LinPublished in: Cell proliferation (2024)
Osteoporosis (OP) is a common disease characterized by bone loss and bone tissue microstructure degradation. Drug treatment is a common clinical treatment that aims to increase bone mass and bone density. Tetrahedral DNA nanostructures (TDNs) are three-dimensional tetrahedral frames formed by folding four single-stranded DNA molecules, which have good biological safety and can promote bone regeneration. In this study, a mouse model of OP was established by ovariectomy (OVX) and TDN was injected into the tail vein for 8 weeks. We found that ovariectomized mice could simulate some physiological changes in OP. After treatment with TDNs, some of this destruction in mice was significantly improved, including an increase in the bone volume fraction (BV/TV) and bone trabecular number (Tb. N), decrease in bone separation (Tb. SP), reduction in the damage to the mouse cartilage layer, reduction in osteoclast lacunae in bone trabecula, and reduction in the damage to the bone dense part. We also found that the expression of ALP, β-Catenin, Runx2, Osterix, and bone morphogenetic protein (BMP)2 significantly decreased in OVX mice but increased after TDN treatment. Therefore, this study suggests that TDNs may regulate the Wnt/β-Catenin and BMP signalling pathways to improve the levels of some specific markers of osteogenic differentiation, such as Runx2, ALP, and Osterix, to promote osteogenesis, thus showing a therapeutic effect on OP mice.
Keyphrases
- bone loss
- bone regeneration
- bone mineral density
- postmenopausal women
- soft tissue
- mouse model
- single molecule
- body composition
- cell proliferation
- epithelial mesenchymal transition
- stem cells
- type diabetes
- high fat diet induced
- cell free
- circulating tumor
- transcription factor
- emergency department
- skeletal muscle
- binding protein
- insulin resistance
- signaling pathway
- electronic health record
- inflammatory response
- lps induced
- molecular dynamics simulations
- long non coding rna
- nucleic acid