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Fourier computed tomographic imaging of two dimensional fluorescent objects.

Patrick A StocktonKeith A WernsingJeffrey J FieldJeff SquierRandy A Bartels
Published in: APL photonics (2019)
We introduce a new form of tomographic imaging that is particularly advantageous for a new class of super-resolution optical imaging methods. Our tomographic method, Fourier Computed Tomography (FCT), operates in a conjugate domain relative to conventional computed tomography techniques. FCT is the first optical tomography method that records complex projections of the object spatial frequency distribution. From these spatial frequency projections, the spatial slice theorem is derived, which is used to build a tomographic imaging reconstruction algorithm. FCT enables enhancement of spatial frequency support along a single spatial direction to be isotropic in the entire transverse spatial frequency domain.
Keyphrases
  • high resolution
  • computed tomography
  • magnetic resonance imaging
  • machine learning
  • mass spectrometry
  • high speed
  • photodynamic therapy
  • fluorescence imaging
  • single molecule
  • fluorescent probe