Adsorption Kinetics of Type-III Antifreeze Protein on Ice Crystal Surfaces.
Dmitry A VorontsovGen SazakiYoshinori FurukawaEvgeniia K TitaevaEkaterina L KimPublished in: Langmuir : the ACS journal of surfaces and colloids (2023)
The spatio-temporal distribution of type-III antifreeze protein (AFP-III) molecules labeled with fluorescent isocyanate (FITC) was visualized at the interfaces between ice and solutions with an FITC-labeled AFP-III (F-AFP-III) concentration of 20-800 μg/mL by fluorescence microscopy. The number density of F-AFP-III on the surface of ice microcrystals was calculated from the calibrated fluorescence intensity. The adsorption of F-AFP-III molecules on the ice crystal surfaces proceeded at a finite rate and then reached the saturation level. The time course of the number density of adsorbed F-AFP-III molecules could be well represented by Langmuir's model. The characteristic adsorption time of F-AFP-III, the adsorption coefficient k 1 = (0.5 ± 0.05) × 10 -4 (μg/mL) -1 s -1 , and the desorption coefficient k 2 = 0.005 ± 0.002 s -1 were determined using the Langmuir's model and obtained experimental data. We found that the adsorption of F-AFP-III could have different kinetics depending on the solution conditions and the type of fluorescence molecules conjugated with AFP-III.
Keyphrases
- type iii
- aqueous solution
- single molecule
- magnetic resonance
- computed tomography
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- magnetic resonance imaging
- photodynamic therapy
- machine learning
- amino acid
- biofilm formation
- mass spectrometry
- binding protein
- optical coherence tomography
- staphylococcus aureus
- quantum dots
- small molecule
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