Effect of the Freezing Step in the Stability and Bioactivity of Protein-Loaded PLGA Nanoparticles Upon Lyophilization.

Pedro FonteFernanda AndradeCláudia AzevedoJoão PintoVítor SeabraMarco van de WeertSalette ReisBruno Sarmento

Published in: Pharmaceutical research (2016)

The freezing process may be detrimental to the structure of protein loaded into nanoparticles, with negative consequences to bioactivity. The co-encapsulation of cryoprotectants mitigated the freezing stresses with benefits to protein bioactivity.

Keyphrases

drug delivery
protein protein
binding protein
cancer therapy
small molecule
recombinant human