LC-MS/MS-based in vitro and in vivo investigation of blood-brain barrier integrity by simultaneous quantitation of mannitol and sucrose.
Behnam NooraniEkram Ahmed ChowdhuryFaleh AlqahtaniYeseul AhnDhavalkumar PatelAbraham Al-AhmadReza MehvarUlrich BickelPublished in: Fluids and barriers of the CNS (2020)
We established a highly sensitive, specific and reproducible approach to simultaneously measure the BBB permeability of two classical low molecular weight, hydrophilic markers in a stable isotope labeled format. This method is now available as a tool to quantify BBB permeability in vitro and in vivo in different disease models, as well as for monitoring treatment outcomes.
Keyphrases
- blood brain barrier
- liquid chromatography
- endothelial cells
- mass spectrometry
- cerebral ischemia
- solid phase extraction
- ms ms
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- pet imaging
- high performance liquid chromatography
- fluorescent probe
- living cells
- simultaneous determination
- positron emission tomography