Visible light-activatable Q-dye molecular beacons for long-term mRNA monitoring in neurons.
Robin KlimekPaul G Donlin-AspClaudio PolisseniVanessa HanffErin M SchumanAlexander HeckelPublished in: Chemical communications (Cambridge, England) (2021)
Herein, we present a new class of Q-dye molecular beacons (MBs) that can be locally activated with visible light in hippocampal neurons. Our novel architecture increases the available monitoring time for neuronal mRNA from several minutes to 14 hours, since a lower light-sampling rate is required for tracking.