A PCR-RFLP method for genotyping of inversion 2Rc in Anopheles coluzzii.

Raquel Montanez-GonzalezAlexandra C ValleraMaria CalzettaVerena PichlerRachel R LoveMoussa W GuelbeogoRoch K DabireMarco PombiCarlo CostantiniFrederic SimardAlessandra Della TorreNora J Besansky

Published in: Parasites & vectors (2021)

We have developed tandem PCR-RFLP assays for the accurate genotyping of inversion 2Rc in An. coluzzii. Because this approach is simple, inexpensive, and requires only basic molecular biology equipment, it is widely accessible. These provide a crucial tool for probing the molecular basis of eco-phenotypes relevant to malaria epidemiology and vector control.

Keyphrases

high throughput
genome wide
contrast enhanced
single molecule
plasmodium falciparum
real time pcr
genetic diversity
risk factors
high resolution
magnetic resonance imaging
dna methylation
molecular dynamics simulations
computed tomography
mass spectrometry
zika virus