Ultrasound-guided hepatic portal vein injection is not a reproducible technique for delivery of cell therapies to the liver in mice.
Sophie L WalkerJune NobleAdrian ThomsonCarmel M MoranDavid MellisI-Ning LeeLisa J WhiteShareen ForbesPublished in: Diabetic medicine : a journal of the British Diabetic Association (2023)
Ultrasound-guided injection results in microparticle clustering in the liver, with an overall reduction in microparticle number when compared to open laparotomy hepatic portal vein injection, and high variability in microparticle-counts detected between mice. Ultrasound-guided injection is not currently a technique that can replace open laparotomy hepatic portal vein injection of islet transplantation in mice.