Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome.

Benjamin Lopez-JimenaStefanie WehnerGraham HaroldMohammed BakheitSieghard FrischmannMichaël BekaertOumar FayeAmadou Alpha SallManfred Weidmann

Published in: PLoS neglected tropical diseases (2018)

The RT-LAMP appeared to show superior performance with material stored for months compared to qRT-PCR and can be therefore recommended for use in infrastructures with poor settings.

Keyphrases

loop mediated isothermal amplification
zika virus
high throughput
aedes aegypti
sensitive detection
room temperature
disease virus