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Fluorescence activated cell sorting-A selective tool for plant cell isolation and analysis.

Ioanna AntoniadiVladimír SkalickýGuiling SunWen MaDavid W GalbraithOndřej NovákKaren Ljung
Published in: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)
Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.
Keyphrases
  • single cell
  • flow cytometry
  • rna seq
  • cell therapy
  • induced apoptosis
  • cell wall
  • healthcare
  • primary care
  • high throughput
  • stem cells
  • mass spectrometry
  • mesenchymal stem cells
  • bone marrow
  • quantum dots