Interaction of a Triantennary Quinoline Glycoconjugate with the Asialoglycoprotein Receptor.
Subhadeep PalitSayanika BanerjeeTridib MahataSougata NiyogiTanusree DasChandra Sova MandiPartha ChakrabartiSanjay DuttaPublished in: ChemMedChem (2021)
Targeted intracellular delivery is an efficient strategy for developing therapeutics against cancer and other intracellular infections. Nonspecific drug delivery shows limited clinical applications owing to high dosage, cytotoxicity, nonspecific action, high cost, etc. Therefore, targeted delivery of less cytotoxic drug candidates to hepatocytes through ASGPR-mediated endocytosis could be an efficient strategy to surmount the prevailing shortcomings. In the present work, the gene encoding ASGPR-H1-CRD was amplified from Huh7 cells, cloned into pET 11a vector, and the ASGPR-H1-CRD protein was expressed and purified from E. coli. A novel triantennary galactose-conjugated quinoline derivative 4 was synthesized that demonstrates 17-fold higher binding affinity to isolated ASGPR-H1-CRD protein receptor (Kd ∼54 μM) in comparison to D-galactose (Kd ∼900 μM). Moreover, micro-calorimetric studies for the interaction of glycoconjugate 4 with ASGPR protein on live hepatocytes showed notable thermal response in case of ASGPR-containing Huh7 cells, in comparison to non-ASGPR Chang cells. These results might serve as an approach towards targeted delivery of small glycoconjugates to hepatocytes.
Keyphrases
- induced apoptosis
- cell cycle arrest
- drug delivery
- binding protein
- endoplasmic reticulum stress
- computed tomography
- escherichia coli
- emergency department
- small molecule
- protein protein
- molecular docking
- cell death
- signaling pathway
- cancer therapy
- oxidative stress
- gene expression
- reactive oxygen species
- pet ct
- pi k akt
- dna methylation
- pet imaging
- lymph node metastasis
- squamous cell