CD11b high B Cells Increase after Stroke and Regulate Microglia.
Janelle M KorfPedram HonarpishehEric C MohanAnik BanerjeeMaria P Blasco-ConesaParisa HonarpishehGary U GuzmanRomeesa KhanBhanu P GaneshAmy L HazenJuneyoung LeeAditya KumarLouise D McCulloughAnjali ChauhanPublished in: Journal of immunology (Baltimore, Md. : 1950) (2022)
Recent studies have highlighted the deleterious contributions of B cells to post-stroke recovery and cognitive decline. Different B cell subsets have been proposed on the basis of expression levels of transcription factors (e.g., T-bet) as well as specific surface proteins. CD11b (α-chain of integrin) is expressed by several immune cell types and is involved in regulation of cell motility, phagocytosis, and other essential functions of host immunity. Although B cells express CD11b, the CD11b high subset of B cells has not been well characterized, especially in immune dysregulation seen with aging and after stroke. Here, we investigate the role of CD11b high B cells in immune responses after stroke in young and aged mice. We evaluated the ability of CD11b high B cells to influence pro- and anti-inflammatory phenotypes of young and aged microglia (MG). We hypothesized that CD11b high B cells accumulate in the brain and contribute to neuroinflammation in aging and after stroke. We found that CD11b high B cells are a heterogeneous subpopulation of B cells predominantly present in naive aged mice. Their frequency increases in the brain after stroke in young and aged mice. Importantly, CD11b high B cells regulate MG phenotype and increase MG phagocytosis in both ex vivo and in vivo settings, likely by production of regulatory cytokines (e.g., TNF-α). As both APCs and adaptive immune cells with long-term memory function, B cells are uniquely positioned to regulate acute and chronic phases of the post-stroke immune response, and their influence is subset specific.
Keyphrases
- immune response
- cognitive decline
- nk cells
- rheumatoid arthritis
- stem cells
- traumatic brain injury
- intensive care unit
- mild cognitive impairment
- hiv infected
- pseudomonas aeruginosa
- toll like receptor
- dendritic cells
- adipose tissue
- multiple sclerosis
- liver failure
- staphylococcus aureus
- cell therapy
- hepatitis b virus
- white matter
- functional connectivity
- african american