Development of single-tube real-time PCR assay for the rapid detection of Aspergillus and Fusarium-The two most common causative agents in fungal keratitis.

Yamini TawdeSourav DasAmit GuptaSavitri SharmaSoham BasakTwishi ShrimaliShreya SinghShivaprakash Mandya RudramurthyHarsimran KaurAnup K Ghosh

Published in: Mycoses (2023)

ITS-2 real-time PCR can be applied as an adjunct with conventional methods for the diagnosis of FK. The genus-specific duplex real-time PCRs are rapid which reduces the turnaround time (TAT) avoiding the need for sequencing.

Keyphrases

real time pcr
cell wall
high throughput
single cell
loop mediated isothermal amplification