Direct measurements of neurosteroid binding to specific sites on GABA A receptors.

Satyanarayana M ChintalaHiroki TateiwaMingxing QianYuanjian XuFatima AmtasharZi-Wei ChenCharles C KirkpatrickJohn BracamontesAllison L GermannGustav AkkDouglas F CoveyAlex S Evers

Published in: British journal of pharmacology (2024)

R were directly measured and an allosteric interaction between the sites was revealed. Adaptation of the MQ290 FRET assay to a plate-reader format will enable screening for high affinity agonists and antagonists for neurosteroid binding sites.

Keyphrases

small molecule
single molecule
high throughput
single cell
living cells
fluorescent probe
energy transfer