Magnetic Graphene Oxide Nanocomposites for Selective miRNA Separation and Recovery.
Supapitch UtenPoramin BoonbanjongYosaphon PrueksathapornKiatnida TreerattrakoonNuankanya SathirapongsasutiNarong ChanlekSupree PinitsoontornPatraporn LuksirikulDeanpen JaprungPublished in: ACS omega (2024)
In this study, we developed magnetic graphene oxide composites by chemically attaching Fe 3 O 4 nanoparticles to graphene oxide nanosheets. Characterization techniques, including Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), Raman spectroscopy, thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), atomic force microscopy (AFM), and transmission electron microscopy (TEM), confirmed the successful synthesis of Fe 3 O 4 @GO composites with desirable properties. The resulting composites exhibited superparamagnetic behavior, solubility, and compatibility for efficient miRNA separation. Using miR-29a as a model, we demonstrated the effective binding of miR-29a to the magnetic graphene oxide (GO) composites at an optimal concentration of 1.5 mg/mL, followed by a simple separation using magnetic forces. Additionally, the addition of 5.0 M urea enhanced the miRNA recovery. These findings highlight the potential use of our magnetic graphene oxide composites for the efficient separation and recovery of miR-29a, suggesting their broad applicability in various miRNA-based studies. Further exploration can focus on investigating endogenous miRNAs with aberrant expression patterns, contributing to the advancements in precision medicine.
Keyphrases
- reduced graphene oxide
- electron microscopy
- molecularly imprinted
- atomic force microscopy
- cell proliferation
- long non coding rna
- raman spectroscopy
- visible light
- high resolution
- gold nanoparticles
- liquid chromatography
- long noncoding rna
- high speed
- single molecule
- mass spectrometry
- magnetic resonance imaging
- risk assessment
- dual energy
- binding protein
- human health
- iron oxide nanoparticles
- solid state