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Highly sensitive Raman measurements of protein aqueous solutions using liquid-liquid phase separation.

Reona TobitaLisa KageyamaAyaka SaitoShinya TaharaShinji KajimotoTakakazu Nakabayashi
Published in: Chemical communications (Cambridge, England) (2024)
A highly sensitive method is proposed for obtaining the Raman spectra of low-concentration proteins and nucleic acids in an aqueous solution using liquid-liquid phase separation. This method uses water droplets formed by adding a large amount of polyethylene glycol into a biomolecular aqueous solution. Ordinary spontaneous Raman spectra are obtained with a high signal-to-noise ratio.
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