Graphene-Layered Eggshell Membrane as a Flexible and Functional Scaffold for Enhanced Proliferation and Differentiation of Stem Cells.
Sunho ParkTeayeop KimYonghyun GwonSujin KimDaun KimHyun-Ha ParkKi-Taek LimHoon Eui JeongKyunghoon KimJangho KimPublished in: ACS applied bio materials (2019)
The eggshell membrane (ESM) is usually regarded as an agricultural byproduct waste, even though it has unique properties as a biomaterial. In particular, the ESM has a flexible and highly pure microfibrous network structure that can be used as an artificial extracellular matrix (ECM) platform for engraftment or as a tissue-engineered scaffold. In this study, flexible and functional scaffolds were constructed using an ESM and graphene, and their applicability for stem cell and tissue engineering was analyzed. The graphene-layered ESM (GEM) scaffolds show enhanced characteristics, such as ECM-like hierarchical micro- and nanostructures and better mechanical and hydrophilic properties than those of a raw ESM. The GEM scaffolds can control the adhesion properties of stem cells, enhancing the proliferation and osteogenic properties of the cells compared with the effects of a raw ESM. Additionally, the GEM scaffolds can improve the secretion of growth factors from stem cells, possibly through enhanced cell-substrate interactions, thereby promoting the proliferation and differentiation of these cells.
Keyphrases
- tissue engineering
- stem cells
- extracellular matrix
- induced apoptosis
- signaling pathway
- cell therapy
- cell cycle arrest
- heavy metals
- mesenchymal stem cells
- risk assessment
- single cell
- bone marrow
- climate change
- carbon nanotubes
- wastewater treatment
- endoplasmic reticulum stress
- pseudomonas aeruginosa
- highly efficient
- reduced graphene oxide
- cell proliferation
- pi k akt
- high throughput
- gold nanoparticles
- staphylococcus aureus
- transition metal
- mass spectrometry
- liquid chromatography
- cell adhesion
- solid state
- network analysis