Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus.
Dmitry S PolyakovEkaterina SinitsynaNatalia GrudininaMariia AntipchikRodion SakhabeevViktor Korzhikov-VlakhMikhail ShavlovskyEvgenia G Korzhikova-VlakhTatiana TennikovaPublished in: Pharmaceutics (2021)
Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro- and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro- and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro- and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.
Keyphrases
- hepatitis c virus
- human immunodeficiency virus
- protein protein
- binding protein
- amino acid
- healthcare
- escherichia coli
- liver failure
- small molecule
- drug induced
- respiratory failure
- combination therapy
- acute respiratory distress syndrome
- nk cells
- extracorporeal membrane oxygenation
- hiv infected
- magnetic nanoparticles
- antiretroviral therapy