The influence of proteoforms: assessing the accuracy of total vitamin D-binding protein quantification by proteolysis and LC-MS/MS.
Lisa E KilpatrickRoger BouillonW Clay DavisClark M HendersonAndrew N HoofnagleSteven PauwelsDirk VanderschuerenEtienne WaelkensHans WildiersJames H YenKaren W PhinneyPublished in: Clinical chemistry and laboratory medicine (2022)
VDBP proteoforms were not found to cause bias during quantification by LC-MS/MS, thus demonstrating that a family of proteins can be accurately quantified using shared peptides. A reference value was assigned for total VDBP in SRM 1950, which may be used to standardize methods and improve the accuracy of VDBP quantification in research and clinical samples.
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