N -Methyl deuterated rhodamines for protein labelling in sensitive fluorescence microscopy.
Kilian RoßmannKerem Can AkkayaPascal PocCorentin CharbonnierJenny EichhorstHannes GonschiorAbha ValavalkarNicolas WendlerThorben CordesBenjamin Dietzek-IvanšićBen J JonesMartin LehmannJohannes BroichhagenPublished in: Chemical science (2022)
Rhodamine fluorophores are setting benchmarks in fluorescence microscopy. Herein, we report the deuterium (d12) congeners of tetramethyl(silicon)rhodamine, obtained by isotopic labelling of the four methyl groups, show improved photophysical parameters ( i.e. brightness, lifetimes) and reduced chemical bleaching. We explore this finding for SNAP- and Halo-tag labelling in live cells, and highlight enhanced properties in several applications, such as fluorescence activated cell sorting, fluorescence lifetime microscopy, stimulated emission depletion nanoscopy and single-molecule Förster-resonance energy transfer. We finally extend this idea to other dye families and envision deuteration as a generalizable concept to improve existing and to develop new chemical biology probes.
Keyphrases
- single molecule
- energy transfer
- quantum dots
- living cells
- atomic force microscopy
- induced apoptosis
- high resolution
- fluorescent probe
- single cell
- hydrogen peroxide
- high throughput
- cell therapy
- small molecule
- cell death
- nitric oxide
- high speed
- cell proliferation
- mass spectrometry
- cell cycle arrest
- highly efficient
- photodynamic therapy
- bone marrow
- mesenchymal stem cells
- pi k akt