A sensitive dual-signal electrochemiluminescence immunosensor based on Ru(bpy) 3 2+ @HKUST-1 and Ce 2 Sn 2 O 7 for detecting the heart failure biomarker NT-proBNP.
Yu JiShuang HeYang ChenPei ZhangJing SunYa LiKaida KuangNengqing JiaPublished in: Journal of materials chemistry. B (2023)
A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy) 3 2+ @HKUST-1/TPA and Ce 2 Sn 2 O 7 /K 2 S 2 O 8 probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy) 3 2+ , effectively improving the anodic signal intensity, while the emerging Ce 2 Sn 2 O 7 emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy. This dual-signal immunosensor has a wide linear range (5 × 10 -4 -1 × 10 4 ng mL -1 ) and a low quantitative detection limit, simultaneously showing high sensitivity, stability and reproducibility, as well as the detection capability of actual serum samples. This dual signal-calibrated immunoassay platform not only reduces the false positive rate of detection results but also provides a promising method for the early diagnosis of heart failure.
Keyphrases
- heart failure
- label free
- energy transfer
- electron microscopy
- sensitive detection
- loop mediated isothermal amplification
- high resolution
- single molecule
- high intensity
- quantum dots
- small molecule
- atrial fibrillation
- solid state
- real time pcr
- living cells
- cardiac resynchronization therapy
- high throughput
- photodynamic therapy
- single cell
- magnetic resonance
- low grade
- risk assessment
- magnetic resonance imaging