Stimulated Raman Scattering Imaging Sheds New Light on Lipid Droplet Biology.
Hao JiaShuhua YuePublished in: The journal of physical chemistry. B (2023)
A lipid droplet (LD) is a dynamic organelle closely associated with cellular functions and energy homeostasis. Dysregulated LD biology underlies an increasing number of human diseases, including metabolic disease, cancer, and neurodegenerative disorder. Commonly used lipid staining and analytical tools have difficulty providing the information regarding LD distribution and composition at the same time. To address this problem, stimulated Raman scattering (SRS) microscopy uses the intrinsic chemical contrast of biomolecules to achieve both direct visualization of LD dynamics and quantitative analysis of LD composition with high molecular selectivity at the subcellular level. Recent developments of Raman tags have further enhanced sensitivity and specificity of SRS imaging without perturbing molecular activity. With these advantages, SRS microscopy has offered great promise for deciphering LD metabolism in single live cells. This article overviews and discusses the latest applications of SRS microscopy as an emerging platform to dissect LD biology in health and disease.
Keyphrases
- high resolution
- high throughput
- single molecule
- label free
- optical coherence tomography
- single cell
- induced apoptosis
- high speed
- endothelial cells
- mental health
- public health
- magnetic resonance
- health information
- oxidative stress
- raman spectroscopy
- magnetic resonance imaging
- photodynamic therapy
- deep learning
- mass spectrometry
- risk assessment
- squamous cell
- artificial intelligence
- social media
- big data
- structural basis
- contrast enhanced
- young adults
- lymph node metastasis
- flow cytometry