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PCR-based detection of the honeybee tracheal mite (Acarapis woodi) in Türkiye.

Rahşan Koç AkpınarAli SevimElif SevimSelma KayaŞakir Önder TürlekCoşkun AydınŞengül Alpay KaraoğluSema Nur ÇelikArif BozdeveciGokhan GuvenBilal KüçükoğluMurat Yaldizİsmail Aydın
Published in: Parasitology research (2023)
Acarapis woodi (Rennie 1921) (Acari: Tarsonemidae) is one of the mites that settles in the respiratory system of honeybees (Apis mellifera L. (Hymenoptera, Apidae)) and distributed throughout the world. It causes significant economic losses on honey production. In Türkiye, studies on the existence of A. woodi are very limited and so far, no studies on the molecular diagnosis and phylogenetic of it have been reported in Türkiye. This study was conducted to investigate the prevalence of A. woodi in Türkiye, especially in areas where beekeeping is intense. Diagnosis of A. woodi was performed using both microscopic and molecular methods using specific PCR primers. Adult honeybee samples were collected from 1.193 hives in 40 provinces of Türkiye between 2018 and 2019. Based on identification studies, the presence of A. woodi was detected in a total of 3 hives (0.5%) in 2018 and 4 hives (0.7%) in 2019. This is the first report for determination of A. woodi in Türkiye.
Keyphrases
  • case control
  • real time pcr
  • risk factors
  • young adults
  • sensitive detection
  • molecularly imprinted
  • simultaneous determination