A NIR fluorescent probe for detection of viscosity and lysosome imaging in live cells.
Tong ChenZikang ChenRuiyuan LiuShaobing ZhengPublished in: Organic & biomolecular chemistry (2019)
Lysosomes, as the cellular recycling center, are filled with numerous hydrolases that can degrade most cellular macromolecules. Studies have shown that the abnormality of viscosity in lysosomes will disrupt the normal function of lysosomes. Herein, a D-π-A structure near-infrared fluorescent probe containing N,N-dimethylamino benzene as an electron donor, benzothiozole as an electron acceptor, and a vinyl group as a π unit, Lyso-BTC, is explored for fluorescence imaging of lysosomes and detection of lysosomal viscosity changes. Lyso-BTC exhibits a large Stokes shift (∼180 nm), NIR emission (685 nm), good biocompatibility, excellent photostability, and fluorescence response to viscosity. Moreover, the results of in vitro studies reveal that Lyso-BTC is lysosome-targeted and could be applied for the detection of viscosity changes in lysosomes caused by chloroquine treatment. These results confirm that Lys-BTC could be employed to monitor lysosomal viscosity changes in living cells.
Keyphrases
- fluorescent probe
- living cells
- fluorescence imaging
- photodynamic therapy
- loop mediated isothermal amplification
- real time pcr
- label free
- high resolution
- induced apoptosis
- single molecule
- single cell
- solar cells
- genome wide
- cancer therapy
- cell death
- drug delivery
- energy transfer
- endoplasmic reticulum stress
- mass spectrometry
- cell proliferation
- combination therapy
- electron transfer