Liver biopsy derived induced pluripotent stem cells provide unlimited supply for the generation of hepatocyte-like cells.
Diego CalabreseGuglielmo RomaSebastian BerglingWalter CarboneValentina MeleSandro NuciforoIsabel FofanaBenedetta CampanaDagmara SzkolnickaDavid C HayJan TchorzTewis BouwmeesterStefan WielandMarkus H HeimPublished in: PloS one (2019)
PLCs and Li-iPSCs obtained from small pieces of human needle liver biopsies constitute a novel unlimited source for the production of HLCs. Despite the preservation of a liver specific gene expression footprint in Li-iPSCs, the generation of fully differentiated hepatocytes cannot be achieved with the current differentiation protocols.