Effect of working gas on physicochemical and biological properties of CaP coatings deposited by RFMS.

Thin calcium phosphate (CaP) coatings were deposited on titanium substrates by radio frequency magnetron sputtering of hydroxyapatite target in the neon (Ne), argon (Ar), krypton (Kr) and xenon (Xe). The influence of the working gas (Ne, Ar, Kr and Xe) on the wettability, biodegradation in the RPMI 1640 synthetic culture medium of the CaP coatings. This paper is the first comprehensive study of working gas effect on properties of the CaP coatings. There was an increase in the polar component of surface free energy (SFE) and a decrease in the dispersion component of SFE with an increase of the atomic mass of the working gas. All CaP coatings had a pronounced protective effect up to 2 times reducing the number of dead cells in culture compared to the Ti control. The most soluble CaP coatings formed in the atmosphere of Xe stimulated the hAMMSCs division, which led to an increase in the total number of cells (208% of the initial culture). Samples with CaP coatings formed in an inert gases atmosphere increased gene expression (ALPL, BMP6, BMP2) in vitro. The most biocompatible coatings were coatings formed in the atmosphere of Xe and Ar.