The Adipose-Derived Stem Cell and Endothelial Cell Coculture System-Role of Growth Factors?
Dominik SteinerHilkea MutschallSophie WinklerRaymund E HorchAndreas ArkudasPublished in: Cells (2021)
Adequate vascularization is a fundamental prerequisite for bone regeneration, formation and tissue engineering applications. Endothelialization of scaffold materials is a promising strategy to support neovascularization and bone tissue formation. Besides oxygen and nutrition supply, the endothelial network plays an important role concerning osteogenic differentiation of osteoprogenitor cells and consecutive bone formation. In this study we aimed to enhance the growth stimulating, proangiogenic and osteogenic features of the ADSC and HUVEC coculture system by means of VEGFA165 and BMP2 application. We were able to show that sprouting phenomena and osteogenic differentiation were enhanced in the ADSC/HUVEC coculture. Furthermore, apoptosis was unidirectionally decreased in HUVECs, but these effects were not further enhanced upon VEGFA165 or BMP2 application. In summary, the ADSC/HUVEC coculture system per se is a powerful tool for bone tissue engineering applications.
Keyphrases
- tissue engineering
- bone regeneration
- cell cycle arrest
- mesenchymal stem cells
- stem cells
- endothelial cells
- induced apoptosis
- bone marrow
- endoplasmic reticulum stress
- cell death
- oxidative stress
- physical activity
- pi k akt
- vascular endothelial growth factor
- bone mineral density
- signaling pathway
- optical coherence tomography
- body composition