Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein.
Carlos Alberto Manuel-CabreraAlba Adriana Vallejo-CardonaEduardo Padilla-CamberosRodolfo Hernández-GutiérrezSara Elisa Herrera-RodríguezAbel Gutiérrez-OrtegaPublished in: Virology journal (2016)
His-TEV CP was purified by immobilized metal affinity chromatography and assembled into VLPs, some of them reaching 2-μm length. TEV VLPs administered along with PRRSV chimeric protein changed the IgG2/IgG1 ratio against the chimeric protein, suggesting that TEV CP can modulate the immune response against a soluble antigen.