Brain Homogenate Decoys for Antigen-Specific Cell Amplification.
J Daniel GriffinSebastian G HuayamaresTowne R WalstonJimmy Y SongMichael ShaoAlexander R SedlacekDeanna L DiazAparna R ChakravartiCory J BerklandPublished in: ACS applied bio materials (2020)
Multiple sclerosis is complex and heterogeneous. Better tools are needed to be able to monitor this disease among individuals, but blood-based biomarkers are often too rare to profile. In this work, we developed antigen-specific biomaterials to replicate the central nervous system niche where multiple sclerosis biomarkers are amplified. We incorporated mouse brain homogenate into a microporous gelatin methacrylate network. Homogenate-containing biomaterials differentially stimulated cells and led to the marked amplification of disease-relevant, antigen-specific B cells. These results demonstrate that biomaterials containing primary tissue homogenate retain antigen specificity and may be a useful tool for decoding human autoimmunity.
Keyphrases
- induced pluripotent stem cells
- multiple sclerosis
- tissue engineering
- bone regeneration
- white matter
- induced apoptosis
- nucleic acid
- cell therapy
- cell cycle arrest
- stem cells
- endothelial cells
- resting state
- cell proliferation
- oxidative stress
- cerebrospinal fluid
- label free
- blood brain barrier
- celiac disease
- structural basis