Aha1 Exhibits Distinctive Dynamics Behavior and Chaperone-Like Activity.
Huifang HuQing WangJingwen DuZhijun LiuYiluan DingHongjuan XueChen ZhouLinyin FengNaixia ZhangPublished in: Molecules (Basel, Switzerland) (2021)
Aha1 is the only co-chaperone known to strongly stimulate the ATPase activity of Hsp90. Meanwhile, besides the well-studied co-chaperone function, human Aha1 has also been demonstrated to exhibit chaperoning activity against stress-denatured proteins. To provide structural insights for a better understanding of Aha1's co-chaperone and chaperone-like activities, nuclear magnetic resonance (NMR) techniques were used to reveal the unique structure and internal dynamics features of full-length human Aha1. We then found that, in solution, both the two domains of Aha1 presented distinctive thermal stabilities and dynamics behaviors defined by their primary sequences and three-dimensional structures. The low thermal stability (melting temperature of Aha128-162: 54.45 °C) and the internal dynamics featured with slow motions on the µs-ms time scale were detected for Aha1's N-terminal domain (Aha1N). The aforementioned experimental results suggest that Aha1N is in an energy-unfavorable state, which would therefore thermostatically favor the interaction of Aha1N with its partner proteins such as Hsp90's middle domain. Differently from Aha1N, Aha1C (Aha1's C-terminal domain) exhibited enhanced thermal stability (melting temperature of Aha1204-335: 72.41 °C) and the internal dynamics featured with intermediate motions on the ps-ns time scale. Aha1C's thermal and structural stabilities make it competent for the stabilization of the exposed hydrophobic groove of dimerized Hsp90's N-terminal domain. Of note, according to the NMR data and the thermal shift results, although the very N-terminal region (M1-W27) and the C-terminal relaxin-like factor (RLF) motif showed no tight contacts with the remaining parts of human Aha1, they were identified to play important roles in the recognition of intrinsically disordered pathological α-synuclein.
Keyphrases
- magnetic resonance
- heat shock protein
- high resolution
- heat shock
- magnetic resonance imaging
- gene expression
- endoplasmic reticulum
- heat stress
- computed tomography
- mass spectrometry
- single cell
- genome wide
- induced pluripotent stem cells
- machine learning
- zika virus
- men who have sex with men
- blood brain barrier
- ionic liquid
- hiv infected
- stress induced