Multispectral near infrared absorption imaging for histology of skin cancer.
Alexander SpreinatGabriele SelvaggioLuise ErpenbeckSebastian KrussPublished in: Journal of biophotonics (2019)
Multispectral imaging combines the spectral resolution of spectroscopy with the spatial resolution of imaging and is therefore very useful for biomedical applications. Currently, histological diagnostics use mainly stainings with standard dyes (eg, hematoxylin + eosin) to identify tumors. This method is not applicable in vivo and provides low amounts of chemical information. Biomolecules absorb near infrared light (NIR, 800-1700 nm) at different wavelengths, which could be used to fingerprint tissue. Here, we built a NIR multispectral absorption imaging setup to study skin tissue samples. NIR light (900-1500 nm) was used for homogenous wide-field transmission illumination and detected by a cooled InGaAs camera. In this setup, images I(x, y, λ) from dermatological samples (melanoma, nodular basal-cell carcinoma, squamous-cell carcinoma) were acquired to distinguish healthy from diseased tissue regions. In summary, we show the potential of multispectral NIR imaging for cancer diagnostics.
Keyphrases
- fluorescence imaging
- high resolution
- photodynamic therapy
- squamous cell carcinoma
- skin cancer
- healthcare
- single molecule
- drug release
- mass spectrometry
- magnetic resonance imaging
- drug delivery
- convolutional neural network
- optical coherence tomography
- computed tomography
- basal cell carcinoma
- high speed
- young adults
- risk assessment
- human health
- wound healing