One-pot synthesis and multiple MS/MS fragmentation studies of phospholysine peptides.
Xuelian ZhaoSongsen FuYufen ZhaoFeng NiPublished in: Rapid communications in mass spectrometry : RCM (2022)
A synthetic method was developed for pLys peptides in aqueous solution within one pot. The characteristic pLys immonium ions and phosphate-related neutral loss could serve as the diagnostic ions for unambiguous phosphosite identification of pLys peptides. In addition, UVPD was promising for the identification of pLys peptides.