Cascade Hydroxyl Radical-Generating and Ferroptosis-Inducing Nanofiber System for the Therapy of Oral Squamous Cell Carcinoma.
JiHye ParkQiaojun HaoDa In JeongHyun-Jin KimSungyun KimSong Yi LeeSeongnam ChuUsok HyunHyun-Jong ChoPublished in: Molecules (Basel, Switzerland) (2024)
Nanofiber (NF) membrane systems that can provide cascade catalytic reaction and ferroptosis induction were developed for oral cancer therapy. Glucose oxidase (GOx) and aminoferrocene (AF) were introduced into the NF system for glucose deprivation/H 2 O 2 generation and OH radical generation, respectively. GOx offers starvation therapy and AF (including iron) provides chemodynamic therapy/ferroptosis for combating oral cancer. GOx (water-soluble) and AF (poorly water-soluble) molecules were successfully entrapped in the NF membrane via an electrospinning process. GOx and AF were incorporated into the polyvinyl alcohol (PVA)-based NF, resulting in PVA/GOx/AF NF with fast disintegration and immediate drug-release properties. In oral squamous cell carcinoma (YD-9 cells), the PVA/GOx/AF NF group exhibited higher cytotoxicity, antiproliferation potential, cellular ROS level, apoptosis induction, lipid ROS level, and malondialdehyde level compared to the other NF groups. The electrospun PVA/GOx/AF NF can be directly applied to oral cancer without causing pain, offering starvation/chemodynamic therapy and ferroptosis induction.
Keyphrases
- signaling pathway
- lps induced
- cell death
- atrial fibrillation
- pi k akt
- oxidative stress
- nuclear factor
- cell cycle arrest
- water soluble
- induced apoptosis
- drug release
- cancer therapy
- drug delivery
- stem cells
- inflammatory response
- type diabetes
- cell proliferation
- toll like receptor
- bone marrow
- adipose tissue
- risk assessment
- blood glucose
- cell therapy
- climate change
- metabolic syndrome
- neuropathic pain
- weight loss
- replacement therapy
- human health
- wound healing