Amplified FRET Nanoflares: An Endogenous mRNA-Powered Nanomachine for Intracellular MicroRNA Imaging.
Jing LiJiaoli WangShiyuan LiuNuli XieKe QuanYanjing YangXiaohai YangJin HuangKemin WangPublished in: Angewandte Chemie (International ed. in English) (2020)
It is of great value to detect biological molecules in live cells. However, probes for imaging low-abundance targets in live cells are limited by the one-to-one signal-triggered model. Here, we introduce the concept of the amplified FRET nanoflare, which employs high-abundance endogenous mRNA as fuel strands to amplify the detection of low abundance intracellular miRNA. As far as we know, this is the first report of an endogenous mRNA-powered nanomachine for intracellular molecular detection. We experimentally prove the mechanism of the nanomachine and demonstrate its specificity and sensitivity. The proposed amplified FRET nanoflare can act as an excellent intracellular molecular detection strategy that is promising for biological and medical applications.
Keyphrases
- single molecule
- induced apoptosis
- living cells
- cell cycle arrest
- reactive oxygen species
- high resolution
- fluorescent probe
- antibiotic resistance genes
- healthcare
- energy transfer
- endoplasmic reticulum stress
- cell death
- fluorescence imaging
- oxidative stress
- signaling pathway
- pi k akt
- photodynamic therapy
- loop mediated isothermal amplification