Chalcogenides-based Tubular Micromotors in Fluorescent Assays.
Víctor de la Asunción-NadalMarta PachecoBeatriz Jurado SánchezAlberto EscarpaPublished in: Analytical chemistry (2020)
WS2/Pt and MoS2/Pt bubble propelled micromotors are used as "on-the-fly" sensing platforms in bioassays, using a highly selective affinity peptide probe for "OFF-ON" detection of Escherichia coli as a model endotoxin. The different outer micromotor surface characteristics play an important role in the sensing performance. The relatively high outer surface of WS2/Pt micromotors results in a 3.5-fold increase in sensitivity compared to MoS2/Pt micromotors due to enhanced peptide probe loading and release. The peptide-modified WS2 micromotors are used as a low-cost and high-throughput approach for the determination of E. coli endotoxin after only 60 s, with a limit of detection of 1.9 ng mL-1 and high selectivity. The method has been validated using spiked samples (tap water, blood serum, and saliva) and bacteria media (blank broth, Staphylococcus aureus, and E. coli). The concept can be extended to the analysis of other (bio)-analytes and easily incorporated into portable instrumentation, holding great promise in a myriad of clinical, environmental, or food-safety applications.
Keyphrases
- quantum dots
- escherichia coli
- low cost
- high throughput
- living cells
- staphylococcus aureus
- label free
- loop mediated isothermal amplification
- biofilm formation
- room temperature
- human health
- fluorescent probe
- big data
- machine learning
- solid phase extraction
- single molecule
- risk assessment
- transition metal
- methicillin resistant staphylococcus aureus
- climate change
- molecularly imprinted
- candida albicans