Iris-derived induced pluripotent stem cells that express GFP in all somatic cells of mice and differentiate into functional retinal neurons.
Noriko HiramatsuNaoki YamamotoMahito OhkumaNoriaki NagaiEi-Ichi MiyachiKumiko YamatsutaKazuyoshi ImaizumiPublished in: Medical molecular morphology (2022)
When regenerated tissue is generated from induced pluripotent stem cells (iPSCs), it is necessary to track and identify the transplanted cells. Fluorescently-labeled iPSCs synthesize a fluorescent substance that is easily tracked. However, the expressed protein should not affect the original genome sequence or pluripotency. To solve this problem, we created a cell tool for basic research on iPSCs. Iris tissue-derived cells from GFP fluorescence-expressing mice (GFP-DBA/2 mice) were reprogrammed to generate GFP mouse iris-derived iPSCs (M-iris GFP iPSCs). M-iris GFP iPSCs expressed cell markers characteristic of iPSCs and showed pluripotency in differentiating into the three germ layers. In addition, when expressing GFP, the cells differentiated into functional recoverin- and calbindin-positive cells. Thus, this cell line will facilitate future studies on iPSCs.
Keyphrases
- induced pluripotent stem cells
- induced apoptosis
- cell cycle arrest
- endoplasmic reticulum stress
- single cell
- oxidative stress
- gene expression
- magnetic resonance imaging
- high fat diet induced
- cell death
- skeletal muscle
- computed tomography
- metabolic syndrome
- cell proliferation
- binding protein
- quantum dots
- pet ct
- wild type
- genome wide