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Authentication Analysis of MT-4 Cells Distributed by the National Institutes of Health AIDS Reagent Program.

Melissa V Fernandez-de CéspedesKrista A Delviks-FrankenberryDavid A ScheiblinChristine HappelVinay K PathakEric O Freed
Published in: Journal of virology (2019)
The MT-4 human T-cell line expresses HTLV-1 Tax and is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. MT-4 cells (lot 150048), distributed by the NIH AIDS Reagent Program (NIH-ARP), were found to be Tax deficient and unable to host replication of the gp41-truncated HIV-1 mutant. These findings, together with short tandem repeat profiling, established that lot 150048 are not bona fide MT-4 cells.
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