Development of Polyelectrolyte Complexes for the Delivery of Peptide-Based Subunit Vaccines against Group A Streptococcus.
Lili ZhaoWanli JinJazmina Gonzalez CruzNirmal MarasiniZeinab G KhalilRobert J CaponWaleed M HusseinMariusz SkwarczynskiIstvan TothPublished in: Nanomaterials (Basel, Switzerland) (2020)
Peptide subunit vaccines hold great potential compared to traditional vaccines. However, peptides alone are poorly immunogenic. Therefore, it is of great importance that a vaccine delivery platform and/or adjuvant that enhances the immunogenicity of peptide antigens is developed. Here, we report the development of two different systems for the delivery of lipopeptide subunit vaccine (LCP-1) against group A streptococcus: polymer-coated liposomes and polyelectrolyte complexes (PECs). First, LCP-1-loaded and alginate/trimethyl chitosan (TMC)-coated liposomes (Lip-1) and LCP-1/alginate/TMC PECs (PEC-1) were examined for their ability to trigger required immune responses in outbred Swiss mice; PEC-1 induced stronger humoral immune responses than Lip-1. To further assess the adjuvanting effect of anionic polymers in PECs, a series of PECs (PEC-1 to PEC-5) were prepared by mixing LCP-1 with different anionic polymers, namely alginate, chondroitin sulfate, dextran, hyaluronic acid, and heparin, then coated with TMC. All produced PECs had similar particle sizes (around 200 nm) and surface charges (around + 30 mV). Notably, PEC-5, which contained heparin, induced higher antigen-specific systemic IgG and mucosal IgA titers than all other PECs. PEC systems, especially when containing heparin and TMC, could function as a promising platform for peptide-based subunit vaccine delivery for intranasal administration.
Keyphrases
- immune response
- hyaluronic acid
- drug delivery
- wound healing
- venous thromboembolism
- growth factor
- high glucose
- diabetic rats
- dendritic cells
- protein kinase
- candida albicans
- toll like receptor
- drug induced
- high throughput
- early stage
- oxidative stress
- type diabetes
- tissue engineering
- skeletal muscle
- inflammatory response
- pseudomonas aeruginosa
- insulin resistance
- cystic fibrosis
- staphylococcus aureus
- high fat diet induced