A major facilitator superfamily domain 8 frameshift variant in a cat with suspected neuronal ceroid lipofuscinosis.
Julien GuevarPetra HugFelix GiebelsAlexane DurandVidhya JagannathanTosso LeebPublished in: Journal of veterinary internal medicine (2019)
A 2-year-old male domestic shorthair cat was presented for a progressive history of abnormal posture, behavior, and mentation. Menace response was absent bilaterally, and generalized tremors were identified on neurological examination. A neuroanatomical diagnosis of diffuse brain dysfunction was made. A neurodegenerative disorder was suspected. Magnetic resonance imaging findings further supported the clinical suspicion. Whole-genome sequencing of the affected cat with filtering of variants against a database of unaffected cats was performed. Candidate variants were confirmed by Sanger sequencing followed by genotyping of a control population. Two homozygous private (unique to individual or families and therefore absent from the breed-matched controlled population) protein-changing variants in the major facilitator superfamily domain 8 (MFSD8) gene, a known candidate gene for neuronal ceroid lipofuscinosis type 7 (CLN7), were identified. The affected cat was homozygous for the alternative allele at both variants. This is the first report of a pathogenic alteration of the MFSD8 gene in a cat strongly suspected to have CLN7.
Keyphrases
- copy number
- genome wide
- genome wide identification
- magnetic resonance imaging
- pulmonary embolism
- cerebral ischemia
- dna methylation
- multiple sclerosis
- healthcare
- computed tomography
- white matter
- oxidative stress
- health insurance
- gene expression
- magnetic resonance
- subarachnoid hemorrhage
- low grade
- single cell
- blood brain barrier
- adverse drug