Preparation and Characterization of Nano-Fe 3 O 4 and Its Application for C18-Functionalized Magnetic Nanomaterials Used as Chromatographic Packing Materials.

A new type of magnetic nanomaterial with Fe 3 O 4 as the core and organic polymer as the shell was synthesized by seed emulsion polymerization. This material not only overcomes the problem of insufficient mechanical strength of the organic polymer, it also solves the problem that Fe 3 O 4 is prone to oxidation and agglomeration. In order to make the particle size of Fe 3 O 4 meet the requirement of the seed, the solvothermal method was used to prepare Fe 3 O 4 . The effects of the reaction time, amount of solvent, pH value, and polyethylene glycol (PEG) on the particle size of Fe 3 O 4 were investigated. In addition, in order to accelerate the reaction rate, the feasibility of preparing Fe 3 O 4 by microwave was studied. The results showed that under the optimum conditions, the particle size of Fe 3 O 4 could reach 400 nm and had good magnetic properties. After three stages of oleic acid coating, seed emulsion polymerization, and C18 modification, the obtained C18-functionalized magnetic nanomaterials were used for the preparation of the chromatographic column. Under optimal conditions, stepwise elution significantly shortened the elution time of sulfamethyldiazine, sulfamethazine, sulfamethoxypyridazine, and sulfamethoxazole while still achieving a baseline separation.