Identification of optimal endogenous reference RNAs for RT-qPCR normalization in hindgut of rat models with anorectal malformations.

Caiyun LongYunxia XiaoSiying LiXiaobing TangZhengwei YuanYu Zuo Bai

Published in: PeerJ (2019)

The most stable reference genes for RT-qPCR were Rpl13a, Ywhaz, and Rps18 in ETU-induced ARMs in rat fetus. This study provided a foundation for reference gene selection for future gene expression analyses.

Keyphrases

gene expression
genome wide
oxidative stress
dna methylation
bioinformatics analysis
genome wide identification
high glucose
current status
drug induced
transcription factor
genome wide analysis
endothelial cells