Raman Spectroscopy Characterization of Multi-Functionalized Liposomes as Drug-Delivery Systems for Neurological Disorders.
Francesca RodàSilvia PiccioliniValentina MangoliniAlice GualerziPierfausto SeneciAntonio RendaSilvia SesanaFrancesca ReMarzia BedoniPublished in: Nanomaterials (Basel, Switzerland) (2023)
The characterization of nanoparticle-based drug-delivery systems represents a crucial step in achieving a comprehensive overview of their physical, chemical, and biological features and evaluating their efficacy and safety in biological systems. We propose Raman Spectroscopy (RS) for the characterization of liposomes (LPs) to be tested for the control of neuroinflammation and microglial dysfunctions in Glioblastoma multiforme and Alzheimer's disease. Drug-loaded LPs were functionalized to cross the blood-brain barrier and to guarantee localized and controlled drug release. The Raman spectra of each LP component were used to evaluate their contribution in the LP Raman fingerprint. Raman data analysis made it possible to statistically discriminate LPs with different functionalization patterns, showing that each molecular component has an influence in the Raman spectrum of the final LP formulation. Moreover, CLS analysis on Raman data revealed a good level of synthetic reproducibility of the formulations and confirmed their stability within one month from their synthesis, demonstrating the ability of the technique to evaluate the efficacy of LP synthesis using small amount of sample. RS represents a valuable tool for a fast, sensitive and label free biochemical characterization of LPs that could be used for quality control of nanoparticle-based therapeutics.
Keyphrases
- raman spectroscopy
- drug release
- drug delivery
- inflammatory response
- data analysis
- label free
- quality control
- anti inflammatory
- lipopolysaccharide induced
- lps induced
- cancer therapy
- quantum dots
- traumatic brain injury
- single cell
- spinal cord
- neuropathic pain
- cognitive impairment
- high resolution
- mild cognitive impairment
- spinal cord injury
- wound healing
- density functional theory
- liquid chromatography
- tandem mass spectrometry
- simultaneous determination