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Evaluation of different storage times and preservation methods on phlebotomine sand fly DNA concentration and purity.

Kamila Gaudêncio da Silva SalesDébora Elienai de Oliveira MirandaFernando José da SilvaDomenico OtrantoLuciana Aguiar FigueredoFilipe Dantas-Torres
Published in: Parasites & vectors (2020)
The preservation methods and storage times herein evaluated did not affect the concentration and purity of DNA samples obtained from field-collected phlebotomine sand flies, for up to 12 months. Furthermore, these preservation methods did not interfere with PCR amplification of CAC and cox1 genes, being suitable for molecular analyses under the conditions studied herein.
Keyphrases
  • single molecule
  • circulating tumor
  • nucleic acid
  • drinking water
  • cell free
  • drosophila melanogaster
  • gene expression
  • solid state
  • label free