A Commercial Non-Binding Surface Effectively Reduces Fibrinogen Adsorption but Does Not Prevent Platelet Adhesion to Fibrinogen.

A commercial non-binding surface effectively prevents protein adsorption, however, the platelet phenotype on this surface has yet to be defined. This study evaluates platelet adhesion and adsorption of several plasma/extra-cellular matrix (ECM) proteins to the non-binding surface compared to other commonly-used non-treated and high-binding surfaces. Platelet adhesion to both uncoated and coated with fibrinogen or collagen microplates was quantified using a colorimetric assay. The binding capacity of the examined surfaces for plasma/ECM proteins was evaluated by measuring the relative and absolute protein adsorption. The non-binding surface effectively prevented platelet adhesion when it was not coated with any protein, resulting in a reduction of up to 92% compared to the non-treated and high-binding surfaces; the non-binding surface also reduced platelet deposition on collagen (up to 31%), but not on fibrinogen. The non-binding surface also exhibited lower adsorption capacity for the examined proteins: 61%-93% reduction in protein adsorption (ELISA) compared to the remaining surfaces. The non-binding surface seems to be more of a low-fouling than non-fouling material, as it was able to reduce fibrinogen adsorption but not prevent platelet adhesion to fibrinogen. This feature should be considered when using the non-binding surface for in vitro platelet testing. This article is protected by copyright. All rights reserved.