Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images.
Jihun KangEun-Hye KangYoung-Shik YunSeungmuk JiIn-Sik YunJong-Souk YeoPublished in: Applied microscopy (2020)
The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections.
Keyphrases
- high resolution
- deep learning
- convolutional neural network
- stem cells
- endothelial cells
- mass spectrometry
- optical coherence tomography
- electron microscopy
- induced apoptosis
- photodynamic therapy
- tandem mass spectrometry
- high speed
- cell cycle arrest
- healthcare
- ionic liquid
- cell death
- endoplasmic reticulum stress
- induced pluripotent stem cells
- mesenchymal stem cells
- liquid chromatography
- high density
- data analysis
- health information