Enhancing suspended cell transfection by inducing localized distribution of the membrane actin cortex before exposure to electromechanical stimulation.
Wenjing HuangYibo MaNaotomo TottoriYoko YamanishiPublished in: Biotechnology letters (2023)
Localized distribution of the actin cortex before exposure to electromechanical stimulation is crucial for inducing a partial loss of the cortex, which improves transfection efficiency and large plasmid delivery.