A Fluorescent Probe for Hydrogen Peroxide in Vivo Based on the Modulation of Intramolecular Charge Transfer.
Yuzhi ChenXiaomin ShiZhengliang LuXuefei WangZhuo WangPublished in: Analytical chemistry (2017)
Endogenous hydrogen peroxide in vivo is related to many diseases, including cancer, diabetes, cardiovascular disease, and neurodegenerative disorders. Although many probes for detection of H2O2 have been explored, rapid response probes are still expected for in vivo application. Here, a new probe (PAM-BN-PB) was designed based on an intramolecular charge transfer (ICT) process with three parts: phenanthroimidazole, benzonitrile, and phenyl boronate. By modulation ICT process of PAM-BN-PB, H2O2 in solution systems can be detected with good selectivity. The exogenous and endogenous H2O2 in normal living cells, ischemia-reperfusion injury cells, and animals all can be imaged by PAM-BN-PB.
Keyphrases
- living cells
- hydrogen peroxide
- fluorescent probe
- cardiovascular disease
- heavy metals
- ischemia reperfusion injury
- nitric oxide
- loop mediated isothermal amplification
- induced apoptosis
- single molecule
- type diabetes
- aqueous solution
- oxidative stress
- cell cycle arrest
- papillary thyroid
- energy transfer
- risk assessment
- squamous cell
- endoplasmic reticulum stress
- glycemic control
- small molecule
- coronary artery disease
- adipose tissue
- cardiovascular risk factors
- insulin resistance
- cell death
- label free
- fluorescence imaging
- sensitive detection
- real time pcr
- weight loss